Harnessing the potential of microalgae-based systems for mitigating pesticide pollution and its impact on their metabolism.

Due to increased pesticide usage in agriculture, a significant concentration of pesticides is reported in the environment that can directly impact humans, aquatic flora, and fauna. Utilizing microalgae-based systems for pesticide removal is becoming more popular because of their environmentally friendly nature, ability to degrade pesticide molecules into simpler, nontoxic molecules, and cost-effectiveness of the technology. Thus, this review focused on the efficiency, mechanisms, and factors governing pesticide removal using microalgae-based systems and their effect on microalgal metabolism. A wide range of pesticides, like atrazine, cypermethrin, malathion, trichlorfon, thiacloprid, etc., can be effectively removed by different microalgal strains. Some species of Chlorella, Chlamydomonas, Scenedesmus, Nostoc, etc., are documented for >90% removal of different pesticides, mainly through the biodegradation mechanism. The antioxidant enzymes such as ascorbate peroxidase, superoxide dismutase, and catalase, as well as the complex structure of microalgae cell walls, are mainly involved in eliminating pesticides and are also crucial for the defense mechanism of microalgae against reactive oxygen species. However, higher pesticide concentrations may alter the biochemical composition and gene expression associated with microalgal growth and metabolism, which may vary depending on the type of strain, the pesticide type, and the concentration. The final section of this review discussed the challenges and prospects of how microalgae can become a successful tool to remediate pesticides.

Antibiotic occurrence, environmental risks, and their removal from aquatic environments using microalgae: Advances and future perspectives.

Antibiotic pollution has caused a continuous increase in the development of antibiotic-resistant bacteria and antibiotic-resistant genes (ARGs) in aquatic environments worldwide. Algae-based bioremediation technology is a promising eco-friendly means to remove antibiotics and highly resistant ARGs, and the generated biomass can be utilized to produce value-added products of industrial significance. This review discussed the prevalence of antibiotics and ARGs in aquatic environments and their environmental risks to non-target organisms. The potential of various microalgal species for antibiotic and ARG removal, their mechanisms, strategies for enhanced removal, and future directions were reviewed. Antibiotics can be degraded into non-toxic compounds in microalgal cells through the action of extracellular polymeric substances, glutathione-S-transferase, and cytochrome P450; however, antibiotic stress can alter microalgal gene expression and growth. This review also deciphered the effect of antibiotic stress on microalgal physiology, biomass production, and biochemical composition that can impact their commercial applications.

Assessing the potential for nevirapine removal and its ecotoxicological effects on Coelastrella tenuitheca and Tetradesmus obliquus in aqueous environment.

Remediation of the antiretroviral (ARV) drug, nevirapine (NVP) has attracted considerable scientific attention in recent years due to its frequent detection and persistence in aquatic environments and potential hazards to living organisms. Algae-based technologies have been emerging as an environmentally friendly option for the removal of pharmaceutical compounds, but their ARV drug removal potential has not been fully explored yet. This study aimed to explore the ecotoxicity and removal potential of NVP by two microalgal species, Coelastrella tenuitheca and Tetradesmus obliquus. Lower environmental concentrations (up to 200 ng L-1) of NVP enhanced the microalgal growth, and the highest dry cell weight of 941.27 mg L-1 was obtained in T. obliquus at 50 ng L-1 NVP concentration. Both microalgae showed varying removal efficiencies (19.53-74.56%) when exposed to NVP concentration levels of up to 4000 ng L-1. At the late log phase (day 8), T. obliquus removed the highest percentage of NVP (74.56%), while C. tenuitheca removed 48% at an initial NVP concentration of 50 ng L-1. Photosynthetic efficiency (Fv/Fm and rETR) of the two microalgal species, however, was not affected by environmental concentrations of NVP (up to 4000 ng L-1) at the mid log phase of growth. SEM analysis demonstrated that both algal species produced distinct ridges on their cell surfaces after NVP uptake. In the ecotoxicity study, the calculated IC50 values of NVP (0-100 mg L-1) after 96 h of exposure were 23.45 mg L-1 (C. tenuitheca) and 18.20 mg L-1 (T. obliquus). The findings of the present study may contribute to a better understanding of the environmental hazards associated with NVP and the efficacy of microalgae in removing this pharmaceutical from aquatic environments.

Insights into the potential impact of algae-mediated wastewater beneficiation for the circular bioeconomy: A global perspective.

Algae-based technologies are one of the emerging solutions to societal issues such as accessibility to clean water and carbon-neutral energy and are a contender for the circular bioeconomy. In this review, recent developments in the use of different algal species for nutrient recovery and biomass production in wastewater, challenges, and future perspectives have been addressed. The ratio and bioavailability of nutrients in wastewater are vital parameters, which significantly impact nutrient recovery efficiency and algal biomass production. However, the optimum nutrient concentration and ratio may vary depending upon the microalgal species as well as cultivation conditions. The use of indigenous algae and algae-based consortia with other microorganisms has been proved promising in improving nutrient recovery efficiency and biomass production in pilot scale operations. However, environmental and cultivation conditions also play a significant role in determining the feasibility of the process. This review further focused on the assessment of the potential benefits of algal biomass production, renewable biofuel generation, and CO2 sequestration using wastewater in different countries on the basis of available data on wastewater generation and estimated nutrient contents. It was estimated that 5-10% replacement of fossil crude requirement with algal biofuels would require ~952-1903 billion m3 of water, 10-21 billion tons of nitrogen, and 2-4 billion tons of phosphorus fertilizers. In this context, coupling wastewater treatment and algal biomass production seem to be the most sustainable option with potential global benefits of polishing wastewater through nutrients recycling and carbon dioxide sequestration.

Exploring nutritional modes of cultivation for enhancing lipid accumulation in microalgae.

The objective of this study was to identify the most promising nutritional mode of growth for enhanced biomass and lipid productivity in a set of twenty microalgal strains, grown under photoautotrophic and mixotrophic/heterotrophic conditions using 2% glucose as carbon source. These included four cyanobacterial strains (Cyanosarcina, Phormidium, Nostoc and Anabaena) and sixteen green algae belonging to six genera (five strains each of Chlorella and Chlorococcum, two of Scenedesmus and one each of Chlamydomonas, Kirchneria, Bracteacoccus and Ulothrix). Lipid productivity ranged from 2-13% under photoautotrophic conditions, 1.7-32% under mixotrophic conditions and 0.9-20% under heterotrophic conditions. MIC-G5 Chlorella sp. followed by MIC-G11 Chlorella sp. exhibited the highest cellular lipid content (355 and 271 µg/ml) and lipid productivity of 32% and 28% respectively in mixotrophic condition. In the glucose supplemented conditions (heterotrophic), a significant reduction in PUFA from 25.1 to 9.4, 29.2 to 12.4 and 44.7 to 10.2 was observed in MIC-G4, MIC-G5 and MIC-G11, respectively. A remarkable enhancement of 33-70% in SFA was recorded under mixotrophic conditions. As the quality of biodiesel is based on high SFA and low PUFA, our results illustrate the significance of glucose supplemented condition as a promising strategy for generating high value biodiesel from algae.

Bioprospecting and indexing the microalgal diversity of different ecological habitats of India.

Our study reports the collection, biodiversity analyses, isolation and identification of microalgae from different habitats of India. Cyanophyceae and Chlorophyceae were the most dominant algal groups recorded, with the highest number being recorded for non-heterocystous cyanobacteria (48), followed by 44 unicellular forms. Sagar Island, Sunderbans recorded the greatest number of algae, and unicellular/colonial green algae were present in all the samples. Shannon's Diversity Index was highest in Koikhali, Sunderbans, followed by Rushikulya River, Odisha. Selective enrichment, purification through serial dilution followed by plating and regular observations led to the isolation of sixteen strains. Identification was done by using microscopic observations, supported with standard monographs and classified as belonging to seven genera (Chlorella, Chlorococcum, Kirchneria, Scenedesmus, Chlamydomonas, Tetracystis and Ulothrix). 18S rDNA sequencing was undertaken for four strains. The set of sixteen strains were screened under standard cultural conditions for their growth kinetics and Chlorella sorokiniana MIC-G5, followed by Chlorella sp. MIC-G4 exhibited the highest growth rates. The strain Chlorococcum sp. MIC-G2 recorded highest chlorophyll, while MIC-G3 ranked highest for carbohydrates. The study aided in identifying the dominant microalgae in the diverse habitats and characterizing their growth rate and carbohydrate content, providing a valuable germplasm for further utilization in agriculture and industry.

Biochemical modulation of growth, lipid quality and productivity in mixotrophic cultures of Chlorella sorokiniana.

The genus Chlorella is a widely employed microalga for biodiesel, as it can be grown using photo/mixo/heterotrophic mode of cultivation. The present investigation was undertaken with the hypothesis that addition of different substrates (amino acids, carbon sources, vitamins) along with reducing agents may aid in diverting Acetyl CoA to malonyl CoA or fatty acid biosynthesis, under mixotrophic conditions in Chlorella sorokiniana. Preliminary investigations undertaken with two reducing agents individually (sodium thiosulphate and methyl viologen) along with selected substrates revealed the promise of sodium thiosulphate (1%) in enhancing lipid accumulation significantly. Further, the role of inclusion of twelve substrates and sodium thiosulphate revealed that supplementation with tryptophan (0.1%) recorded 57.28% enhancement in lipid productivity on 4(th) day. Highest values of lipid productivity of 33% were recorded on 8(th) day in 0.1% glucose supplemented medium containing sodium thiosulphate. Fatty Acid Methyl Ester (FAME) profiles generated revealed significant reduction in the content of Poly unsaturated fatty acids (PUFA) and enhanced Mono unsaturated fatty acids (MUFA) (especially oleic acid) in the treatments involving tryptophan, Vitamin B12, sodium pyruvate and glucose. This study reveals the promise of using sodium thiosulphate along with selected substrate for enriching the quality and quantity of lipids, which can be valuable for exploiting algae as a source of biodiesel.

Algal diversity in flowing waters at an acidic mine drainage "barrens" in central Pennsylvania, USA.

Microscopic investigations were undertaken to decipher the diversity in the lotic algal communities from acidic waters (pH 2.4-3.2) flowing overland in sheets and channels at an acid mine drainage (AMD) barrens near Kylertown, PA, USA. Microscopic observations, supplemented with taxonomic keys, aided in identification of the dominant algae, and measurement of carbon from adjacent soils was undertaken. The unicellular protist Euglena sp. was most abundant in slower flowing waters (i.e., pool near point of emergence and surficial flow sheets), while Ulothrix sp. was most abundant in faster flowing water from the central stream channel. A diverse range of unicellular microalgae such as Chlorella, Cylindrocystis, Botryococcus, and Navicula and several filamentous forms identified as Microspora, Cladophora, and Binuclearia were also recorded. The observed high algal diversity may be related to the long duration of AMD flow at this site which has led to the development of adapted algal communities. The comparatively higher carbon content in soil materials adjacent to slower flowing water sampling locations provides evidence for the important role of algae as primary producers in this extreme environment.